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Ligands Raise the Constraint That Limits Constitutive Activation in G Protein-coupled Opioid Receptors
Using a cell-free bioluminescence resonance energy transfer strategy we compared the levels of spontaneous and ligand-induced receptor-G protein coupling in δ (DOP) and μ (MOP) opioid receptors. In this assay GDP can suppress spontaneous coupling, thus allowing its quantification. The level of const...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3745342/ https://ncbi.nlm.nih.gov/pubmed/23836900 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.474452 |
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