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A continuous fluorescence assay for the characterization of Nudix hydrolases

The common substrate structure for the functionally diverse Nudix protein superfamily is nucleotide-diphosphate-X, where X is a large variety of leaving groups. The substrate specificity is known for less than 1% of the 29,400 known members. Most activities result in the release of an inorganic phos...

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Autors principals: Xu, Anting, Desai, Anna M., Brenner, Steven E., Kirsch, Jack F.
Format: Artigo
Idioma:Inglês
Publicat: Academic Press 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3744803/
https://ncbi.nlm.nih.gov/pubmed/23481913
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.02.023
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