Effective Alu Repeat Based RT-Qpcr Normalization in Cancer Cell Perturbation Experiments

BACKGROUND: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories. A specific set of mRNAs as internal control reference genes is considered as the preferred strategy to normalize RT-qPCR data. P...

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Main Authors: Rihani, Ali, Van Maerken, Tom, Pattyn, Filip, Van Peer, Gert, Beckers, Anneleen, De Brouwer, Sara, Kumps, Candy, Mets, Evelien, Van der Meulen, Joni, Rondou, Pieter, Leonelli, Carina, Mestdagh, Pieter, Speleman, Frank, Vandesompele, Jo
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2013
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3743747/
https://ncbi.nlm.nih.gov/pubmed/23977142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0071776
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