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Effective Alu Repeat Based RT-Qpcr Normalization in Cancer Cell Perturbation Experiments

BACKGROUND: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories. A specific set of mRNAs as internal control reference genes is considered as the preferred strategy to normalize RT-qPCR data. P...

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Detalhes bibliográficos
Main Authors: Rihani, Ali, Van Maerken, Tom, Pattyn, Filip, Van Peer, Gert, Beckers, Anneleen, De Brouwer, Sara, Kumps, Candy, Mets, Evelien, Van der Meulen, Joni, Rondou, Pieter, Leonelli, Carina, Mestdagh, Pieter, Speleman, Frank, Vandesompele, Jo
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3743747/
https://ncbi.nlm.nih.gov/pubmed/23977142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0071776
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