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A Novel High Throughput Biochemical Assay to Evaluate the HuR Protein-RNA Complex Formation
The RNA binding protein HuR/ELAVL1 binds to AU-rich elements (AREs) promoting the stabilization and translation of a number of mRNAs into the cytoplasm, dictating their fate. We applied the AlphaScreen technology using purified human HuR protein, expressed in a mammalian cell-based system, to charac...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Public Library of Science
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3741180/ https://ncbi.nlm.nih.gov/pubmed/23951323 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0072426 |
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