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Strategies for isolating constitutively-active and dominant-negative pheromone receptor mutants in yeast

Mating pheromone receptors of the yeast Saccharomyces cerevisiae are useful models for the study of G protein-coupled receptors. The mating pheromone receptors, Ste2 and Ste3, are not essential for viability so they can be readily targeted for analysis by a variety of genetic approaches. This chapte...

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Detalhes bibliográficos
Main Authors: Dosil, Mercedes, Konopka, James B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3741071/
https://ncbi.nlm.nih.gov/pubmed/21050926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-381296-4.00019-1
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