Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling.

The STE4 gene, which encodes the beta subunit of the mating response G-protein in the yeast Saccharomyces cerevisiae, was subjected to a saturation mutagenesis using 'doped' oligodeoxynucleotides. We employed a genetic screen to select dominant-negative STE4 mutants, which when overexpress...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Leberer, E, Dignard, D, Hougan, L, Thomas, D Y, Whiteway, M
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC556956/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1464310/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1992.tb05586.x
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