A New Method for Stranded Whole Transcriptome RNA-seq

This report describes an improved protocol to generate stranded, barcoded RNA-seq libraries to capture the whole transcriptome. By optimizing the use of duplex specific nuclease (DSN) to remove ribosomal RNA reads from stranded barcoded libraries, we demonstrate improved efficiency of multiplexed ne...

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Bibliographic Details
Main Authors: Miller, David F.B., Yan, Pearlly S., Buechlein, Aaron, Rodriguez, Benjamin A., Yilmaz, Ayse S., Goel, Shokhi, Lin, Hai, Collins-Burow, Bridgette, Rhodes, Lyndsay V., Braun, Chris, Pradeep, Sunila, Rupaimoole, Rajesha, Dalkilic, Mehmet, Sood, Anil K., Burow, Matthew E., Tang, Haixu, Huang, Tim H., Liu, Yunlong, Rusch, Douglas B., Nephew, Kenneth P.
Format: Artigo
Language:Inglês
Published: 2013
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3739992/
https://ncbi.nlm.nih.gov/pubmed/23557989
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2013.03.023
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