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A New Method for Stranded Whole Transcriptome RNA-seq
This report describes an improved protocol to generate stranded, barcoded RNA-seq libraries to capture the whole transcriptome. By optimizing the use of duplex specific nuclease (DSN) to remove ribosomal RNA reads from stranded barcoded libraries, we demonstrate improved efficiency of multiplexed ne...
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| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3739992/ https://ncbi.nlm.nih.gov/pubmed/23557989 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2013.03.023 |
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