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Density-dependent cooperative non-specific binding in solid-phase SELEX affinity selection
The non-specific binding of undesired ligands to a target is the primary factor limiting the enrichment of tight-binding ligands in affinity selection. Solution-phase non-specific affinity is determined by the free-energy of ligand binding to a single target. However, the solid-phase affinity might...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3737557/ https://ncbi.nlm.nih.gov/pubmed/23737446 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt477 |
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