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Generating in vitro transcripts with homogenous 3′ ends using trans-acting antigenomic delta ribozyme
In most in vitro run-off transcription reactions with T7 RNA polymerase, transcripts with heterogeneous ends are commonly obtained. Towards the goal of finding a simple and effective procedure for correct processing of their 3′ ends we propose the use of trans-acting antigenomic delta ribozyme. We d...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2004
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373431/ https://ncbi.nlm.nih.gov/pubmed/14973333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh037 |
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