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Generating in vitro transcripts with homogenous 3′ ends using trans-acting antigenomic delta ribozyme

In most in vitro run-off transcription reactions with T7 RNA polymerase, transcripts with heterogeneous ends are commonly obtained. Towards the goal of finding a simple and effective procedure for correct processing of their 3′ ends we propose the use of trans-acting antigenomic delta ribozyme. We d...

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Main Authors: Wichłacz, Agnieszka, Łęgiewicz, Michał, Ciesiołka, Jerzy
Formáid: Artigo
Teanga:Inglês
Foilsithe: Oxford University Press 2004
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC373431/
https://ncbi.nlm.nih.gov/pubmed/14973333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh037
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