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Generating in vitro transcripts with homogenous 3′ ends using trans-acting antigenomic delta ribozyme

In most in vitro run-off transcription reactions with T7 RNA polymerase, transcripts with heterogeneous ends are commonly obtained. Towards the goal of finding a simple and effective procedure for correct processing of their 3′ ends we propose the use of trans-acting antigenomic delta ribozyme. We d...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Wichłacz, Agnieszka, Łęgiewicz, Michał, Ciesiołka, Jerzy
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2004
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373431/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14973333/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh037
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