Generating in vitro transcripts with homogenous 3′ ends using trans-acting antigenomic delta ribozyme
In most in vitro run-off transcription reactions with T7 RNA polymerase, transcripts with heterogeneous ends are commonly obtained. Towards the goal of finding a simple and effective procedure for correct processing of their 3′ ends we propose the use of trans-acting antigenomic delta ribozyme. We d...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373431/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14973333/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh037 |
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