Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution
Sequence saturation mutagenesis (SeSaM) is a conceptually novel and practically simple method that truly randomizes a target sequence at every single nucleotide position. A SeSaM experiment can be accomplished within 2–3 days and comprises four steps: generating a pool of DNA fragments with random l...
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| Опубликовано в:: | Nucleic Acids Res |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2004
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373423/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14872057/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh028 |
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