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Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution
Sequence saturation mutagenesis (SeSaM) is a conceptually novel and practically simple method that truly randomizes a target sequence at every single nucleotide position. A SeSaM experiment can be accomplished within 2–3 days and comprises four steps: generating a pool of DNA fragments with random l...
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| Hlavní autoři: | , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
2004
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373423/ https://ncbi.nlm.nih.gov/pubmed/14872057 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh028 |
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