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PCR amplification of DNA containing non-standard base pairs by variants of reverse transcriptase from Human Immunodeficiency Virus-1

As the next step towards generating a synthetic biology from artificial genetic information systems, we have examined variants of HIV reverse transcriptase (RT) for their ability to synthesize duplex DNA incorporating the non-standard base pair between 2,4-diaminopyrimidine (pyDAD), a pyrimidine pre...

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Detalhes bibliográficos
Main Authors: Sismour, A. Michael, Lutz, Stefan, Park, Jeong-Ho, Lutz, Michael J., Boyer, Paul L., Hughes, Stephen H., Benner, Steven A.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC373358/
https://ncbi.nlm.nih.gov/pubmed/14757837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh241
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