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Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1.

We constructed a series of BspMI cassettes that simplify the introduction of specific point mutations in the polymerase domain of human immunodeficiency virus type 1 reverse transcriptase. A series of point mutants were constructed by using these cassette vectors. The RNA-dependent DNA polymerase an...

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Autores principales: Boyer, P L, Ferris, A L, Hughes, S H
Formato: Artigo
Lenguaje:Inglês
Publicado: 1992
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC240806/
https://ncbi.nlm.nih.gov/pubmed/1370546
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