Identifying ribozyme-accessible sites using NUH triplet-targeting gapmers

Accurately identifying accessible sites in RNA is a critical prerequisite for optimising the cleavage efficiency of hammerhead ribozymes and other small nucleozymes. Here we describe a simple RNase H-based procedure to rapidly identify hammerhead ribozyme-accessible sites in gene length RNAs. Twelve...

Descripció completa

Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Mir, Alain A., Lockett, Trevor J., Hendry, Philip
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 2001
Matèries:	Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC37256/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11328874/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.9.1906
Etiquetes:	Afegir etiqueta Sense etiquetes, Sigues el primer a etiquetar aquest registre!