Identifying ribozyme-accessible sites using NUH triplet-targeting gapmers
Accurately identifying accessible sites in RNA is a critical prerequisite for optimising the cleavage efficiency of hammerhead ribozymes and other small nucleozymes. Here we describe a simple RNase H-based procedure to rapidly identify hammerhead ribozyme-accessible sites in gene length RNAs. Twelve...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC37256/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11328874/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.9.1906 |
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