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Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment
Obtaining NMR assignments for slowly tumbling molecules such as detergent-solubilized membrane proteins is often compromised by low sensitivity as well as spectral overlap. Both problems can be addressed by amino-acid specific isotope labeling in conjunction with (15)N–(1)H correlation experiments....
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3725308/ https://ncbi.nlm.nih.gov/pubmed/22252484 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-012-9601-1 |
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