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Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles

Optimization of aqueous solutions of the integral membrane protein (IMP) OmpW for NMR structure determination has been monitored with micro-coil NMR, which enables the acquisition of NMR spectra using only micrograms of protein and detergent. The detergent 30-Fos (2-undecylphosphocholine) was found...

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Библиографические подробности
Главные авторы: Stanczak, Pawel, Zhang, Qinghai, Horst, Reto, Serrano, Pedro, Wüthrich, Kurt
Формат: Artigo
Язык:Inglês
Опубликовано: 2012
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3715323/
https://ncbi.nlm.nih.gov/pubmed/22890565
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-012-9658-x
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