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Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles

Optimization of aqueous solutions of the integral membrane protein (IMP) OmpW for NMR structure determination has been monitored with micro-coil NMR, which enables the acquisition of NMR spectra using only micrograms of protein and detergent. The detergent 30-Fos (2-undecylphosphocholine) was found...

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Bibliographische Detailangaben
Hauptverfasser: Stanczak, Pawel, Zhang, Qinghai, Horst, Reto, Serrano, Pedro, Wüthrich, Kurt
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3715323/
https://ncbi.nlm.nih.gov/pubmed/22890565
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-012-9658-x
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