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Alkyne lipids as substrates for click chemistry-based in vitro enzymatic assays

Click chemistry is evolving as a powerful tool in biological applications because it allows the sensitive and specific detection of compounds with alkyne or azido groups. Here we describe the use of alkyne lipids as substrates for in vitro enzymatic assays of lipid modifying enzymes. The small alkyn...

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Hlavní autoři: Gaebler, Anne, Milan, Robin, Straub, Leon, Hoelper, Dominik, Kuerschner, Lars, Thiele, Christoph
Médium: Artigo
Jazyk:Inglês
Vydáno: The American Society for Biochemistry and Molecular Biology 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3708378/
https://ncbi.nlm.nih.gov/pubmed/23709689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D038653
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