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A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins

The demand to study the cellular localization of specific lipids has led to recent advances in lipid probes and microscopy. Alkyne lipids bear a small, noninterfering tag and can be detected upon click reaction with an azide-coupled reporter. Fluorescent alkyne lipid imaging crucially depends on app...

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Dades bibliogràfiques
Publicat a:J Lipid Res
Autors principals: Gaebler, Anne, Penno, Anke, Kuerschner, Lars, Thiele, Christoph
Format: Artigo
Idioma:Inglês
Publicat: The American Society for Biochemistry and Molecular Biology 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5036357/
https://ncbi.nlm.nih.gov/pubmed/27565170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D070565
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