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Alkyne lipids as substrates for click chemistry-based in vitro enzymatic assays

Click chemistry is evolving as a powerful tool in biological applications because it allows the sensitive and specific detection of compounds with alkyne or azido groups. Here we describe the use of alkyne lipids as substrates for in vitro enzymatic assays of lipid modifying enzymes. The small alkyn...

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Detalhes bibliográficos
Main Authors: Gaebler, Anne, Milan, Robin, Straub, Leon, Hoelper, Dominik, Kuerschner, Lars, Thiele, Christoph
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3708378/
https://ncbi.nlm.nih.gov/pubmed/23709689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D038653
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