Alkyne lipids as substrates for click chemistry-based in vitro enzymatic assays

Click chemistry is evolving as a powerful tool in biological applications because it allows the sensitive and specific detection of compounds with alkyne or azido groups. Here we describe the use of alkyne lipids as substrates for in vitro enzymatic assays of lipid modifying enzymes. The small alkyn...

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Bibliografiske detaljer
Main Authors: Gaebler, Anne, Milan, Robin, Straub, Leon, Hoelper, Dominik, Kuerschner, Lars, Thiele, Christoph
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Biochemistry and Molecular Biology 2013
Fag:
Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3708378/
https://ncbi.nlm.nih.gov/pubmed/23709689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D038653
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