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Measuring how much work the chaperone GroEL can do
Noncovalently “stacked” tetramethylrhodamine (TMR) dimers have been used to both report and perturb the allosteric equilibrium in GroEL. A GroEL mutant (K242C) has been labeled with TMR, close to the peptide-binding site in the apical domain, such that TMR molecules on adjacent subunits are able to...
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
2013
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3703975/ https://ncbi.nlm.nih.gov/pubmed/23723348 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1307837110 |
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