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Measuring how much work the chaperone GroEL can do

Noncovalently “stacked” tetramethylrhodamine (TMR) dimers have been used to both report and perturb the allosteric equilibrium in GroEL. A GroEL mutant (K242C) has been labeled with TMR, close to the peptide-binding site in the apical domain, such that TMR molecules on adjacent subunits are able to...

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Bibliographic Details
Main Authors: Corsepius, Nicholas C., Lorimer, George H.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2013
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3703975/
https://ncbi.nlm.nih.gov/pubmed/23723348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1307837110
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