Optimization of the β-Elimination/Michael Addition Chemistry on Reversed-Phase Supports for Mass Spectrometry Analysis of O-Linked Protein Modifications

We previously adapted the β-elimination/Michael addition chemistry to solid-phase derivatization on reversed-phase supports, and demonstrated the utility of this reaction format to prepare phosphoseryl peptides in unfractionated protein digests for mass spectrometric identification and facile phosph...

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Bibliografiske detaljer
Main Authors: Nika, Heinz, Nieves, Edward, Hawke, David H., Angeletti, Ruth Hogue
Format: Artigo
Sprog:Inglês
Udgivet: Association of Biomolecular Resource Facilities 2013
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3703673/
https://ncbi.nlm.nih.gov/pubmed/23997661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7171/jbt.13-2403-005
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