High contrast en bloc staining of neuronal tissue for field emission scanning electron microscopy

Conventional heavy metal post staining methods on thin sections lend contrast but often cause contamination. To avoid this problem, we tested several en bloc staining techniques to contrast tissue in serial sections mounted on solid substrates for examination by Field Emission Scanning Electron Micr...

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Bibliografiske detaljer
Main Authors: Tapia, Juan C., Kasthuri, Narayanan, Hayworth, Kenneth, Schalek, Richard, Lichtman, Jeff W., Smith, Stephen J, Buchanan, JoAnn
Format: Artigo
Sprog:Inglês
Udgivet: 2012
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3701260/
https://ncbi.nlm.nih.gov/pubmed/22240582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.439
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