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Large-volume en-bloc staining for electron microscopy-based connectomics
Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Pub. Group
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4532871/ https://ncbi.nlm.nih.gov/pubmed/26235643 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms8923 |
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