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Fitness Loss and Library Size Determination in Saturation Mutagenesis
Saturation mutagenesis is a widely used directed evolution technique, in which a large number of protein variants, each having random amino acids in certain predetermined positions, are screened in order to discover high-fitness variants among them. Several metrics for determining the library size (...
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Public Library of Science
2013
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3700877/ https://ncbi.nlm.nih.gov/pubmed/23844158 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0068069 |
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