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Fitness Loss and Library Size Determination in Saturation Mutagenesis

Saturation mutagenesis is a widely used directed evolution technique, in which a large number of protein variants, each having random amino acids in certain predetermined positions, are screened in order to discover high-fitness variants among them. Several metrics for determining the library size (...

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Bibliografische gegevens
Hoofdauteur: Nov, Yuval
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2013
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3700877/
https://ncbi.nlm.nih.gov/pubmed/23844158
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0068069
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