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Saturation mutagenesis reveals manifold determinants of exon definition
To illuminate the extent and roles of exonic sequences in the splicing of human RNA transcripts, we conducted saturation mutagenesis of a 51-nt internal exon in a three-exon minigene. All possible single and tandem dinucleotide substitutions were surveyed. Using high-throughput genetics, 5560 minige...
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| Publicado no: | Genome Res |
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| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5749175/ https://ncbi.nlm.nih.gov/pubmed/29242188 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.219683.116 |
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