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Enhanced Yield of Recombinant Proteins with Site-Specifically Incorporated Unnatural Amino Acids Using a Cell-Free Expression System

Using a commercial protein expression system, we sought the crucial elements and conditions for the expression of proteins with genetically encoded unnatural amino acids. By identifying the most important translational components, we were able to increase suppression efficiency to 55% and to increas...

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מידע ביבליוגרפי
Main Authors: Smolskaya, Sviatlana, Zhang, Zhiwen Jonathan, Alfonta, Lital
פורמט: Artigo
שפה:Inglês
יצא לאור: Public Library of Science 2013
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3699557/
https://ncbi.nlm.nih.gov/pubmed/23844190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0068363
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