Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency.
We have developed a procedure that gives a very high efficiency of transfection in mammalian cells with low-molecular-weight DNA (approximately 10(4) base pairs). The procedure uses cells in suspension that are shocked with polyethylene glycol 4 h after replating. We compared this transfection techn...
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| Published in: | Mol Cell Biol |
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| Main Authors: | , , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Taylor & Francis
1982
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6294505/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.2.9.1145-1154.1982 |
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