Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency.

We have developed a procedure that gives a very high efficiency of transfection in mammalian cells with low-molecular-weight DNA (approximately 10(4) base pairs). The procedure uses cells in suspension that are shocked with polyethylene glycol 4 h after replating. We compared this transfection techn...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Mol Cell Biol
Main Authors: Shen, Y M, Hirschhorn, R R, Mercer, W E, Surmacz, E, Tsutsui, Y, Soprano, K J, Baserga, R
Format: Artigo
Jezik:Inglês
Izdano: Taylor & Francis 1982
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369908/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6294505/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.2.9.1145
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