Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency.

We have developed a procedure that gives a very high efficiency of transfection in mammalian cells with low-molecular-weight DNA (approximately 10(4) base pairs). The procedure uses cells in suspension that are shocked with polyethylene glycol 4 h after replating. We compared this transfection techn...

Celý popis

Podrobná bibliografie

Vydáno v:	Mol Cell Biol
Hlavní autoři:	Shen, Y M, Hirschhorn, R R, Mercer, W E, Surmacz, E, Tsutsui, Y, Soprano, K J, Baserga, R
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Taylor & Francis 1982
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6294505/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.2.9.1145-1154.1982
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!