General method for cloning amplified DNA by differential screening with genomic probes.

Mutant Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate, a potent and specific inhibitor of aspartate transcarbamylase, have amplified the gene coding for the multifunctional protein (CAD) that includes this activity. The average amount of DNA amplified is approximately 500 kilo...

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Bibliographic Details
Main Authors: Brison, O, Ardeshir, F, Stark, G R
Format: Artigo
Language:Inglês
Published: 1982
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC369828/
https://ncbi.nlm.nih.gov/pubmed/6896736
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