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Major Groove Substituents and Polymerase Recognition of a Class of Predominantly Hydrophobic Unnatural Base Pairs

Expansion of the genetic alphabet with an unnatural base pair is a long-standing goal of synthetic biology. We have developed a class of unnatural base pairs, formed between d5SICS and analogues of dMMO2, that are efficiently and selectively replicated by the Klenow Fragment (Kf) DNA polymerase. In...

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Main Authors: Lavergne, Thomas, Malyshev, Denis A., Romesberg, Floyd E.
Format: Artigo
Jezik:Inglês
Izdano: 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3693734/
https://ncbi.nlm.nih.gov/pubmed/22190386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.201102066
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