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Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm

Quantitative studies in molecular and structural biology generally require accurate and precise determination of protein concentrations, preferably via a method that is both quick and straightforward to perform. The measurement of ultraviolet absorbance at 280 nm has proven especially useful, since...

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Detalhes bibliográficos
Main Authors: Anthis, Nicholas J, Clore, G Marius
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Publishing Ltd 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3690723/
https://ncbi.nlm.nih.gov/pubmed/23526461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2253
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