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The Reactant State for Substrate-Activated Turnover of Acetylthiocholine by Butyrylcholinesterase is a Tetrahedral Intermediate
Secondary β-deuterium kinetic isotope effects have been measured as a function of substrate concentration for recombinant human butyrylcholinesterase-catalyzed hydrolysis of acetyl-L(3)-thiocholine (L = (1)H or (2)H). The isotope effect on V/K is inverse, (D3)V/K = 0.93 ± 0.03, which is consistent w...
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| Autors principals: | , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2005
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3685723/ https://ncbi.nlm.nih.gov/pubmed/16231883 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja052401q |
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