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Expression and amplification of engineered mouse dihydrofolate reductase minigenes.

We constructed mouse dihydrofolate reductase (DHFR) minigenes (dhfr) that had 1.5 kilobases of 5' flanking sequences and contained either none or only one of the intervening sequences that are normally present in the coding region. They were greater than or equal to 3.2 kilobase long, about one-tent...

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Bibliographic Details
Published in:Mol Cell Biol
Main Authors: Crouse, G F, McEwan, R N, Pearson, M L
Format: Artigo
Language:Inglês
Published: Taylor & Francis 1983
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368530/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6835212/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.3.2.257-266.1983
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