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Optimization of harvesting, extraction, and analytical protocols for UPLC-ESI-MS-based metabolomic analysis of adherent mammalian cancer cells

In this study, a liquid chromatography mass spectrometry (LC/MS)-based metabolomics protocol was optimized for quenching, harvesting, and extraction of metabolites from the human pancreatic cancer cell line Panc-1. Trypsin/ethylenediaminetetraacetic acid (EDTA) treatment and cell scraping in water w...

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Hlavní autoři: Bi, Huichang, Krausz, Kristopher W., Manna, Soumen K., Li, Fei, Johnson, Caroline H., Gonzalez, Frank J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3678261/
https://ncbi.nlm.nih.gov/pubmed/23604415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-013-6927-9
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