QuaNCAT: quantitating proteome dynamics in primary cells

Here we demonstrate that quantitation of stimuli-induced proteome dynamics in primary cells is feasible by combining the power of Bio-Orthogonal Non Canonical Amino acid Tagging (BONCAT) and Stable Isotope Labelling of Amino acids in Cell culture (SILAC). In conjunction with nanoLC-MS/MS QuaNCAT all...

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Autors principals: Howden, Andrew J.M., Geoghegan, Vincent, Katsch, Kristin, Efstathiou, Georgios, Bhushan, Bhaskar, Boutureira, Omar, Thomas, Benjamin, Trudgian, David C., Kessler, Benedikt M., Dieterich, Daniela C., Davis, Benjamin G., Acuto, Oreste
Format: Artigo
Idioma:Inglês
Publicat: 2013
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3676679/
https://ncbi.nlm.nih.gov/pubmed/23474466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2401
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