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QuaNCAT: quantitating proteome dynamics in primary cells
Here we demonstrate that quantitation of stimuli-induced proteome dynamics in primary cells is feasible by combining the power of Bio-Orthogonal Non Canonical Amino acid Tagging (BONCAT) and Stable Isotope Labelling of Amino acids in Cell culture (SILAC). In conjunction with nanoLC-MS/MS QuaNCAT all...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3676679/ https://ncbi.nlm.nih.gov/pubmed/23474466 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2401 |
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