Stable conditional expression and effect of C/EBPβ-LIP in adipocytes using the pSLIK system

Murine 3T3-L1 adipocytes are widely used as a cellular model of obesity. However, whereas transfection of 3T3-L1 preadipocytes is straightforward, ectopic gene expression in mature 3T3-L1 adipocytes has proved challenging. Here, we used the pSLIK vector system to generate stable doxycycline-inducibl...

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Detalhes bibliográficos
Main Authors: Esteves, Cristina L, Kelly, Val, Bégay, Valérie, Lillico, Simon G, Leutz, Achim, Seckl, Jonathan R, Chapman, Karen E
Formato: Artigo
Idioma:Inglês
Publicado em: Society for Endocrinology 2013
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3672996/
https://ncbi.nlm.nih.gov/pubmed/23620165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1530/JME-13-0029
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