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Rapid enrichment of HeLa transcription factors IIIB and IIIC by using affinity chromatography based on avidin-biotin interactions.

Plasmid DNA containing the adenovirus type 2 genes for VA RNA was linearized at a site distal to the gene, end labeled with a biotin-nucleotide analog of TTP, and incubated with avidin to form an avidin-biotinylated DNA complex. HeLa cell S100 extracts containing crude RNA polymerase III and transcr...

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Detalhes bibliográficos
Publicado no:Mol Cell Biol
Principais autores: Kasher, M S, Pintel, D, Ward, D C
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367046/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3785224/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.9.3117-3127.1986
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