Rapid enrichment of HeLa transcription factors IIIB and IIIC by using affinity chromatography based on avidin-biotin interactions.

Plasmid DNA containing the adenovirus type 2 genes for VA RNA was linearized at a site distal to the gene, end labeled with a biotin-nucleotide analog of TTP, and incubated with avidin to form an avidin-biotinylated DNA complex. HeLa cell S100 extracts containing crude RNA polymerase III and transcr...

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Hlavní autoři: Kasher, M S, Pintel, D, Ward, D C
Médium: Artigo
Jazyk:Inglês
Vydáno: 1986
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC367046/
https://ncbi.nlm.nih.gov/pubmed/3785224
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