Rapid enrichment of HeLa transcription factors IIIB and IIIC by using affinity chromatography based on avidin-biotin interactions.
Plasmid DNA containing the adenovirus type 2 genes for VA RNA was linearized at a site distal to the gene, end labeled with a biotin-nucleotide analog of TTP, and incubated with avidin to form an avidin-biotinylated DNA complex. HeLa cell S100 extracts containing crude RNA polymerase III and transcr...
Guardat en:
| Publicat a: | Mol Cell Biol |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis
1986
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367046/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3785224/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.9.3117-3127.1986 |
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