Rapid enrichment of HeLa transcription factors IIIB and IIIC by using affinity chromatography based on avidin-biotin interactions.

Plasmid DNA containing the adenovirus type 2 genes for VA RNA was linearized at a site distal to the gene, end labeled with a biotin-nucleotide analog of TTP, and incubated with avidin to form an avidin-biotinylated DNA complex. HeLa cell S100 extracts containing crude RNA polymerase III and transcr...

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Bibliografiske detaljer

Udgivet i:	Mol Cell Biol
Principais autores:	Kasher, M S, Pintel, D, Ward, D C
Format:	Artigo
Sprog:	Inglês
Udgivet:	Taylor & Francis 1986
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367046/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3785224/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.9.3117-3127.1986
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