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Alternative Computational Protocols for Supercharging Protein Surfaces for Reversible Unfolding and Retention of Stability

Reengineering protein surfaces to exhibit high net charge, referred to as “supercharging”, can improve reversibility of unfolding by preventing aggregation of partially unfolded states. Incorporation of charged side chains should be optimized while considering structural and energetic consequences,...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Der, Bryan S., Kluwe, Christien, Miklos, Aleksandr E., Jacak, Ron, Lyskov, Sergey, Gray, Jeffrey J., Georgiou, George, Ellington, Andrew D., Kuhlman, Brian
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3669367/
https://ncbi.nlm.nih.gov/pubmed/23741319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0064363
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