Purification and characterization of human H-ras proteins expressed in Escherichia coli.

The full-length normal and T24 mutant human H-ras proteins and two truncated derivatives of the T24 mutant were expressed efficiently in Escherichia coli. The proteins accumulated to 1 to 5% of total cellular protein, and each was specifically recognized by anti-ras monoclonal antibodies. The two fu...

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Publicado en:	Mol Cell Biol
Autores principales:	Gross, M, Sweet, R W, Sathe, G, Yokoyama, S, Fasano, O, Goldfarb, M, Wigler, M, Rosenberg, M
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Taylor & Francis 1985
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC366817/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3923330/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.5.1015-1024.1985
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