Purification and characterization of human H-ras proteins expressed in Escherichia coli.

The full-length normal and T24 mutant human H-ras proteins and two truncated derivatives of the T24 mutant were expressed efficiently in Escherichia coli. The proteins accumulated to 1 to 5% of total cellular protein, and each was specifically recognized by anti-ras monoclonal antibodies. The two fu...

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Autors principals: Gross, M, Sweet, R W, Sathe, G, Yokoyama, S, Fasano, O, Goldfarb, M, Wigler, M, Rosenberg, M
Format: Artigo
Idioma:Inglês
Publicat: 1985
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC366817/
https://ncbi.nlm.nih.gov/pubmed/3923330
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